Indian Journal of Plant Genetic Resources

  • Year: 2011
  • Volume: 24
  • Issue: 3

Genetic Diversity Analysis of Antirrhinum (Antirrhinum majus L.) Inbreds Using RAPD Markers

  • Author:
  • VK Singh1,, S Tewari1, R Kaushal1, G Bangari2, P Kumar3
  • Total Page Count: 6
  • DOI:
  • Page Number: 299 to 304

1Department of Genetics and Plant Breeding, GB Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand

2Department of Plant Pathology, GB Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand

3Department of Horticulture, GB Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand

Abstract

Genetic diversity analysis of 15 Antirrhinum majus L. inbreds was carried out using RAPD markers to assess genetic diversity present amongst them. A total of 80 DNA amplicons were scored using nine primers. Inbreds shared 63% genetic similarity among themselves. Inbreds were distinguishable from each other based on their banding pattern which is evident by the fact that none of the inbreds shared 100% similarity between each other. UPGMA cluster analysis grouped the inbreds into four clusters at 85% similarity. The co-phenetic correlation coefficient (r) was 0.9322, exhibiting best fit between dendrogram and similarity matrix based on RAPD data. The polymorphism information content (PIC) was in the range of 0.00 to 0.962, while average expected gene diversity ranged from 0.00 to 0.425. Jaccard's pair wise similarity coefficient ranged from 55 to 98.8%.

Keywords

Antirrhinum, Cluster, Genetic diversity, Polymorphism information content, RAPD