Development of Microsatellite Markers from Enriched Genomic Library of ISH 100 (Saccharum hybrid) for Genetic Variability of Sugarcane

Microsatellite primers were developed from the interspecific hybrid ISH 100 of sugarcane to study patterns of population genetic structure across the species. About 26 SSr primers were screened, out of which 13 exhibited polymorphism (50%). These 13 SSr primers were assessed on 8 individuals sampled from one population. The number of alleles per locus ranged from 2 to 6 and observed heterozygosity ranged from 0.16 to 0.60. The genetic diversity among the population depicted from this study can be used for genetic diversity analysis of sugarcane.