1Department of Genetics and Plant Breeding, Raj Mohini Devi College of Agriculture and Research Station, Indira Gandhi Krishi Vishwavidyalaya, Ambikapur-497001, Chhattisgarh, India
3Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, Raipur-492012, Chhattisgarh, India
*corresponding author: E-mail: tiwarijk5@gmail.com
Online published on 23 September, 2015.
Molecular markers are useful tools for determining cultivar identity. The purpose of this study to establish fingerprints of four rice varieties in Chhattisgarh. Microsatellite combines several features of an ultimate molecular marker and they are being used increasingly in various plant genetic studies and applications. The varieties were evaluated utilizing 115 microsatellite markers distributed over the whole rice genome. A total of 160 alleles were detected using 24 microsatellite primer-pairs, and the number of alleles/marker ranged from 2 to 4 with an average of 2.95 alleles/locus. The result suggested that a relatively rapid and small number of microsatellite markers could be used for the identification of rice varieties.
DNA Fingerprinting, Markers Rice, Microsatellite, Variety identification
