Indian Journal of Plant Genetic Resources
  • Year: 2016
  • Volume: 29
  • Issue: 2

Population Structure and Genetic Variation in Indian and African Eleusine coracana (L.) Gaertn

1Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110012, India

2Crop Science Division, Indian Council of Agricultural Research, Krishi Bhavan, New Delhi-110001, India

3Emeritus Scientist, All India Coordinated Small Millet Improvement Project (AICSMIP), Bangalore-560065, India

*Author for Correspondence: Email- lalitnbpgr@rediffmail.com

Online published on 20 October, 2016.

Abstract

Population structure and genetic variation was estimated for 40 Indian and African finger millet genotypes using 237 random amplified polymorphic DNA (RAPD) and 16 morphological markers revealing differences between African and Indian groups. African genotypes showed 53.59%; 0.17±0.19; 0.27±0.27 compared to 48.10%; 0.15±0.19; 0.24±0.27 polymorphism, Nei's gene diversity and Shannon's Information index, respectively in Indian genotypes. Primers OPM-20, OPW-04, OPX-11 were identified as the most informative primers in terms of high % polymorphism and Nei's gene diversity values. Population structure analysis revealed the existence of two distinct sub-groups, correlated well with primary and secondary centers of finger millet diversity and can be utilized to enhance and widen finger millet germplasm base.

Keywords

Finger millet, RAPD, Genetic diversity, Population structure