Indian Journal of Plant Genetic Resources
  • Year: 2016
  • Volume: 29
  • Issue: 2

Analysis of Genetic Diversity in Alpinia galanga using ISSR Markers

1Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore-560089, Karnatanka, India

2School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram-695016, Kerala, India

*Author for Correspondence: Email- rajasekharan.pe@gmail.com

Online published on 20 October, 2016.

Abstract

Genetic diversity analysis was carried out in Alpinia galanga accessions collected from Kerala state of India using Inter Simple Sequence Repeats (ISSR) markers. A total of 60 bands were generated from 18 accessions of A. galanga using 11 ISSR primers. Of these 24 (40%) were polymorphic and remaining 36 (60%) were monomorphic. Total Nei's genetic diversity among the three populations was estimated as h= 0.1735 ± 0.013 and I= 0.2470 ± 0.078. Nei's estimator of population substructure (GST) indicated a fairly low level of population differentiation (GST = 0.2139). Based on this study we propose a conservation strategy to this species.

Keywords

Alpinia galanga, Genetic differentiation, Genetic diversity, Greater galangal, ISSR