Indian Journal of Plant Genetic Resources
  • Year: 2017
  • Volume: 30
  • Issue: 2

Estimation of Genetic Diversity among the Rice (Oryza sativa L.) Genotypes using Microsatellite Markers

1Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India

2Department of Botany, Institute of Science, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India

*Author for Correspondence: Email-pksbhu@gmail.com

Online published on 21 September, 2017.

Abstract

Sixteen microsatellite markers were used to study the genetic diversity among 73 genotypes of rice from geographically diverse locations. Thirteen markers revealed polymorphism, while three were observed to show the monomorphic amplicons. The polymorphic markers produced total of 34 alleles with an average of 2.62 alleles per locus. Amplification products ranged from 100 bp produced by marker K39512 to 220 bp produced by marker RM536. Polymorphism Information Content (PIC) value varied from 0.225 to 0.743 and the marker RM336 was observed to be most suitable for discriminating the genotypes owing to its highest PIC value. The UPGMA cluster diagram grouped the genotypes into eight major clusters. The Jaccard's similarity coefficients ranged from 0.16 to 1.00.

Keywords

Dendrogram, Diversity, Microsatellite markers, PIC value, Rice