1ICAR-National Bureau of Plant Genetic Resources, Pusa campus, New Delhi-110012, India
2Present Address: Division of Crop Improvement, ICAR-Central Research Institute for Jute and Allied Fibres, Kolkata, West Bengal, India
3Punjab Agricultural University, Ludhiana-141004, India
*Author for Correspondence: Email- Ishwari.Bisht@icar.gov.in
Online published on 7 February, 2019.
A total of 38 finger millet genotypes including 18 released varieties and 20 landraces from India were evaluated and compared based on RAPD, ISSR markers and morphological data collectively. RAPD markers showed higher polymorphism (61.62%) as compared to ISSR (57.00%) markers. The mean number of bands per primer and Jaccard's similarity coefficients generated for the markers RAPD, ISSR was 12.9; 0.810 and 14.3; 0.782 respectively. UPGMA clustering, and Structure analysis placed the landraces and varieties in separate groups with some exceptions. The data for 13 qualitative traits revealed diversity mainly for ear shape and ear size. ANOVA analysis of the 14 quantitative traits showed most of the characters to have signifi cant difference, representing the presence of genotypic differences among the 38 genotypes studied. The morpho-variability as well as variation based on molecular markers was higher in the landraces compared to the varieties. The usage of more diverse landraces as genitors to augment the genetic base and identify genes for adaptive and nutritional variations as well as conservation of more landraces to maximize the available genetic diversity of finger millet was emphasized.
ISSR, Landraces, Morphological, RAPD, Varieties
