Indian Journal of Plant Genetic Resources

  • Year: 2019
  • Volume: 32
  • Issue: 1

Molecular Characterization of Oilseed Brassica Reference Set for Stem Rot Disease

  • Author:
  • VV Singh, Priyamedha, Pankaj Sharma, Monika Dubey, Pawan Paliwal, Pankaj Garg, Reema Rani, PK Rai
  • Total Page Count: 8
  • Page Number: 85 to 92

ICAR-Directorate of Rapeseed-Mustard Research, Sewar, Bharatpur-321303 (Rajasthan), India

Abstract

Present study was conducted on oilseed brassica reference set covering resistance and susceptible genotypes (41 Brassica juncea, two Brassica carinata, one Sinapis alba) to gain the information on extent of genetic diversity using 30 polymorphic Simple Sequence Repeat (SSR) markers. The dendrogram obtained by the UPGMA analysis of 82 polymorphic loci amplified using 30 polymorphic SSR markers, resulted in defi nite groupings among the reference set. The polymorphic information content (PIC) values varied from 0.23 to 0.67 with an average of 0.53. The highest PIC value was observed for marker named BRMS-054 making it most informative marker. The polymorphic SSR markers identified in the present study can be used for characterizing Brassica cultivars, constructing database, tagging genes/QTL governing stem rot tolerance and ultimately in identifying markers for the disease.

Keywords

Brassica, Genetic diversity, Polymorphic information content (PIC), SSR markers, Sclerotinia stem rot