1Centre for Agricultural Bioinformatics, ICAR-Indian Agricultural Statistics Research Institute (IASRI), Library Avenue, Pusa Campus, New Delhi-110012, India
2ICAR-National Bureau Plant Genetic Resources, Pusa Campus, New Delhi-110012, India
*Author for Correspondence: Email- d.wankhede@icar.gov.in
Online published on 24 June, 2022.
Dolichos bean (Lablab purpureus L.) is an old cultivated pulse of Africa, Asia and Australia. In spite of its multiple uses, there are limited genomic resources available, which could facilitate genetic diversity studies and DNA fingerprinting. Here, we report the extraction of SSR markers from the Expressed Sequence Tags (ESTs) of dolichos bean. From a total 1129 unique ESTs of dolichos bean available in public domain, a total of 83 SSRs were identified. Among these EST-SSRs, tri-nucleotide repeats were most abundant (37) followed by tetra-nucleotide repeats (18) and di-nucleotide repeats (15). Functionality of the novel SSRs were validated by successful PCR amplification in nine varieties of dolichos bean using ten SSR markers. The novel EST-SSR markers are expected to be helpful in genetic diversity studies as well as for varietal DNA fingerprinting in this important legume.
Dolichos bean, Expressed sequence tags (ESTs), Polymorphic Simple sequence repeats (SSRs)
