Indian Journal of Plant Genetic Resources
  • Year: 2025
  • Volume: 38
  • Issue: 2

A Novel Loop-Mediated Isothermal Amplification Assay as a Potential Method for Varietal Discrimination-A Case Study in Rice

1Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012, India

2Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

*Author for correspondence: sheel.y85@gmail.com

Online published on 11 March, 2026.

Abstract

A novel loop-mediated isothermal amplification (LAMP) assay was developed to distinguish authentic Basmati rice varieties from non-Basmatis, based on 10 bp insertion-deletion (InDel) identified in the Lax1 gene. The developed assay demonstrated high specificity and allowed discrimination between Basmati and adulterant non-Basmatis with a detection limit of 10%.

Keywords

Basmati rice, Adulteration, LAMP, InDel, Promoter