Biochemical changes and conservation of watermelon (Citrullus lantaus thunb.) germplasm

Watermelon (Citrullus lanatus Thunb.) germplasm is commonly preserved in the form of seeds, which is simple, inexpensive and practical means of conservation. Watermelon seeds are short lived for two years under ambient conditions. Seeds of cv Arka Manik having 8.0 per cent moisture were packed in polyethylene (200 gauge) and aluminium foil laminated bags (paper-foil-polyethylene) and stored at ambient (16–35°C), low (5°C) and sub-zero (−20°C) temperatures. With the low temperature storage of seeds, the longevity was extended to S years without decline in seedling vigour. Both polyethylene and laminated bags were effective for retaining the Viability for S years at 5°C and -20°C. Biochemical changes were studied in stored seeds, as a result there was increased leaching of electrolytes, soluble sugars and free amino acids and decreased in dehydrogenase activity on deterioration. Storage of seeds in polyethylene or laminated bags at 5°C or -20°C was effective in conserving germplasm in the form of seeds for five years without decline in vigour.