Procedure for in vitro multiplication and in vitro conservation of six threatened/endangered medicinal plants are reported. Various combinations of growth regulators were tested to select optimal medium for initiation and further shoot multiplication. Slow growth experiments were performed and the shelf-life of shoot cultures on multiplication and/or modified medium could be extended for 11–20 months depending on the species.
In-vitro conservation, Colens forshohlii, Gentiana kurroo, Picrorhiza hurroa, Rauvollia serpentina, Sausseona lappa, Tylphora indica
