National Plant Tissue Culture Repository, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110 012.
In vitro shoot multiplication of Orchis latifolia, a threatened medicinal orchid has been achieved on MS medium supplemented with BAP (1.0 mg/l) and NAA (0.1 mg/l) using sprouting buds as explants. Occasionally “protocorm” like structures were also produced on the same medium: The in vitro raisd shoots could be successfully conserved for more than 10 months at 10°C without Intermittent subculture.
Salam Panja, Orchis latifolia, orchid, in vitro multiplication, in vitro conservation
