Centre for Biosciences, Jamia Millia Islamia, New Delhi 110 025.
Esterase and acid-phosphatase isozyme patterns were studied at different developmental stages (0hr, 24 hrs, 48hrs, 96hrs, 120hrs, 144hrs) in four cultivars of Lens culinaris Medik) The results of this study show that the total enzyme repertory of an organism changes during development and differentiation which can be detected by the expression of their isozymes at the various developmental stages which are under genetic regulatory control mechanisms. In accordance with this a characteristic isozyme pattern was found in the different varieties of Lens culinaris at different developmental stages. Plant populations can also be differentiated genetically as was studied by observing the degree of polymorphism with respect to these two isozymes.
Lens culinaris, isozyme, PAGE, esterascs, acid-phosphatascs