1Department of Medical Laboratory Technology, Prince Fahd Bin Sultan Research chair, Faculty of Applied Medical Sciences
2Department of Clinical Biochemistry, Faculty of Medicine, University of Tabuk
3Department of Biology, Faculty of Science, Al Hussein Bin Tala University, Jordan
4Department of Cardiology, North West Armed Forces Hospital, Tabuk, Kingdom of Saudi Arabia
Online published on 21 February, 2019.
Cardiac troponin T (TNNT2) mutations have been associated with Dilated cardiomyopathy (DCM) and overall frequency of TNNT2 mutations in familial DCM is approximately 3–6%. Therefore, we investigated the incidence of TNNT2 gene variation in Tabuk population.
Study was conducted on 103 subjects including 36 smokers 67 nonsmokers. Genotyping of the TNNT2-rs3729547T>C was performed by using Allele specific PCR method (AS-PCR). The frequency of TNNT2-rs3729547T>C gene polymorphism was determined and its correlation with smokers and nonsmokers was performed. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were applied to evaluate the correlation of this TNNT2-rs3729547T>C polymorphisms with smokers and nonsmokers.
A significant difference was observed in the genotype distribution TNNT2 (rs3729547 T>C) among the Smokers and nonsmokers (p=0.0003). The frequencies of all three genotypes TT, CT, CC reported in the smoker samples were 64%, 13% and 0% and in the nonsmoker samples were 76%, 24% and 0% respectively. Genotypes of TNNT2-rs3729547 CT (OR = 1.80, 95% CI, 0.74–4.35; RR 1.24(0.86–1.79, p=0.22) TNNT2 (TT+ CT) (OR = 1.19, 95% CI, 0.62–2.25; RR 1.05(0.86–1.30, p=0.58), TNNT2 (TT) (OR = 0.90, 95% CI, 0.50–1.61; RR 0.96(0.80–1.16, p=0.72) were not significantly correlated with smokers and nonsmokers.
A significant correlation was found between Cardiac Troponin T (TNNT2) (rs3729547 T>C) gene polymorphism among smokers and nonsmokers of Tabuk population of Saudi Arabia.
Cardiac Troponin T (TNNT2), AS-PCR-allele specific PCR, OR-Odds ratioa
