Molecular & Antibiotic Sensitivity Pattern Study of Acute Otitis Media, Tonsillitis & Periodontitis among Different Ages of Patients of Al-Hashmia General Hospital, Primary Care Centers & Private Clinic in Babylon Province

A total of 89 specimens have included 34 swabs of periodontitis(13male, 21 female), 28 swabs of Otitis Media & 27 swabs of Tonsillitis. These samples were brought from patients consulted to Alhashmia Hospital, primary care centers & private clinic of Babylon province during the period from 5 October 2017 to 11December 2017. They were appeared (72.17)% positive cases for Streptococcus pyogenes which have gotten from patients within age ranged, which suffered from Ear infection (3 to 39 years), Tonsillitis(1 to 60 years) & periodontitis inflammation(from less than 3years to 60 years). Biochemical examinations have included oxidase, catalase & Antibiotics sensitivity test. Results of bacterial genetics detection of sic as responsible for virulence agents of isolates bacteria, which is responsible for damage host cell components. 50 patterns (55.3%) of S. pyogenes cases of otitis media, tonsillitis & gingivitis have positive exclude 39(44.7) isolates were negative on 3% agarose gel. The volume of sic gene was 150 bp of the DNA, ladder (100–1000)bp. This gene of S. pyogenes specimens has amplified by PCR utilizing specific forward & reverse primers have appeared that 50 Streptococcus isolates detection of Otitis Media., Tonsillitis & periodontitis infections have negative 27 (90%) except 2 (10%) isolates were positive.