1Ecological Sciences, AL-Qasim Green University, Hillah City, Iraq
2Department of Biology, College of Science for Women, University of Babylon, Hillah City, Iraq
3Biomedical Science Department University of Babylon, College of Nursing, Hillah City, Iraq
Online published on 21 February, 2019.
Vitex agnus-castus is a supplement derived from berries. It is also called Vitex, Chaste Tree, or Chasteberry. The objectives of this study were analysis of the secondary metabolite products using Fourier-transform infrared spectroscopic profile and evaluation of its anti-microbial activity. In the current study, the anti-microbial activity of Vitex agnus-castus and Citrus sinensis methanolic extract was evaluated by determining the zone of inhibition against nine bacteria and eight fungi and yeast. Maximum zone formation was against E.coli (5.26±0.19) and very highly active against Aspergillus terreus (5.01±0.17). The FTIR analysis of Vitex agnus-castus proved the presence of Alkenes, alkyl halides, and Aromatic which shows major peaks at 715.59, 1014.56, 1047.35, 1095.57, 1234.44, 1242.16, 1317.38 and 1597.06. The FTIR analysis of Citrus sinensis proved the presence of alkyl halides, Alkenes, alkyl halides, and Amide which shows major peaks at 675.09, 692.44, 738.74, 813.96, 974.05, 1008.77, 1049.28, 1093.64, 1232.51, 1276.88, 1606.70, and 1647.21
Vitex agnus-castus, Citrus sinensis, FT-IR, Anti-Bacterial, Anti-Fungal Activity
