1Senior Lecturer, Department of Orthodontics and Dentofacial Orthopaedics, A.B. Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be) University, Mangalore, India
2Professor, PG Department of Biotechnology, Department of Research in Nanotechnology, ALVAS College Moodabidri, Karnataka
3Professor, Department of Microbiology, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore
4Professor, Department of Orthodontics and Dentofacial Orthopaedics, A.B. Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be University), Mangalore, India
*Corresponding Author: P.S. Murali, Senior Lecturer, Department of Orthodontics and Dentofacial Orthopaedics, A.B. Shetty Memorial Institute of Dental Sciences, NITTE (Deemed to be University), Mangalore, India, e-mail: drmuralips@agmail.com, 9746344566
Online published on 23 December, 2019.
The aim of the present study was to assess the antimicrobial activity of orthodontic adhesive mixed with silver nanoparticles (SNPs) in varying concentrations.
SNPs were prepared by using the chemical reduction method and characterized using UV-Vis Spectrophotometer, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy. The synthesized SNPs were mixed with commercially available orthodontic adhesive TransbondTM XT in varying concentrations (1%, 5% and 10%, n = 20 each) w/w and were formed into disc shaped specimens and incubated with Streptococcus mutans MTCC 497 and Lactobacillus acidophilus MTCC10307. The Disc Diffusion test was performed to examine the growth inhibition zones. Adhesive discs without silver nanoparticles were used as control (n = 20). The data was interpreted statistically with Kruskal-Wallis test followed by Tukeys Post Hoc analysis and Mann Whitney U test. P < 0.05 was considered statistically significant.
The characterization of reduced silver ions by UV spectroscopy showed surface plasma resonance band around 400nm. SEM study showed particles with roughly spherical shape with less than 100 nm size. The present study showed that the orthodontic bonding adhesive with SNPs showed higher antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus as compared to the control group even in low concentration of 1% (P < 0.05). The adhesive mixed with SNPs in varying concentrations exhibited higher zone of inhibition in Streptococcus mutans compared with Lactobacillus acidophilus (P < 0.05).
The present study showed that the orthodontic bonding adhesive with silver nanoparticles showed excellent antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus even in low concentrations.
Antimicrobial, Lactobacillus acidophilus, nanoparticles, orthodontic, Streptococcus mutans
