Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 10

Role of p53 and p21 Polymorphisms in Cervical Cancer Risk among Rural Population of South-Western Maharashtra: A Case-Control Study

  • Author:
  • Kailas D. Datkhile1,, Madhavi N. Patil2, Pratik P. Durgawale3, Anand Gudur4, Rashmi A. Gudur5
  • Total Page Count: 5
  • Page Number: 277 to 281

1Senior Research Officer, Department of Molecular Biology & Genetics, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara

2Junior Research Officer, Department of Oncology, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara

3Research Officer, Department of Oncology, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara

4Professor, Department of Oncology, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara

5Medical Oncologist, Department of Oncology, Krishna Institute of Medical Sciences “Deemed to be University”, Taluka-Karad, Dist-Satara

*Corresponding Author: Dr. Kailas D. Datkhile Department of Molecular Biology & Genetics, Krishna Institute of Medical Sciences “Deemed to be University ”Karad, Satara, Maharashtra Tel No: 02164-241555/56, e-mail: hodgeneticslab@kimsuniversity.in

Online published on 23 December, 2019.

Abstract

Earlier discrepancies in the risk of cervical carcinogenesis due to p53 and p21 genes influenced us to elucidate their association with cervical cancer (CC) in the rural population of south-western Maharashtra.

The hospital based case-control study aimed to investigate association of polymorphisms in p21, p53 genes with risk of CC in Maharashtrian population.

PCR-RFLP method was used to genotype polymorphisms in exon 4 and exon 7 of p53, exon 2 and exon 3 of p21 gene from 350 CC patients and 400 controls.

The variant allele Ser/Ser showed association with increased risk CC as compared to Arg/Arg genotype of codon 249 of p53 whereas codon 72 was not associated risk of CC in rural population. Similarly when we studied C/T and T/T genotypes of exon 3 of p21, T/T genotypes showed significant risk of CC (OR=6.03, 95% CI: 2.63–13.81; p<0.0001).

The findings from this study showed possible association of p53 codon 249 and p21 exon3 polymorphisms with increased risk of cervical carcinogenesis in the women of rural population of Maharashtra.

Keywords

Cervical Cancer, PCR-RFLP, p53, Genetic Polymorphism