Histopathological Changes of Mice Immunized by Serratia Marcescens Whole Cell Sonicated Antigens

The research was designed to prepare sonicated antigen of Serratiamarcescens and evaluate its histopathological effect of mice organs which include (liver, spleen, lung and kidney). For this purpose, 24 mice randomly divided into four groups. The first group was immunized with killed whole cell sonicated Serratiamarcescens (KWCSA-SM) (1000 μg/ml) subcutaneously. Second group was immunized with killed whole cell sonicated S.marcescens(KWCSA-SM) (500 μg/ml) subcutaneously. Third group was immunized with whole cell S.marcescens (1000cfu/ml) subcutaneously, 4th group was injected by P.B.S.(pH7.2) as negative control group. Histopathological changes many showed differences between the first group which characterized by sever lesion and other immunized groups, compared with the control group which showed mild lesion in their organs which include severe mononuclear cells infiltration in the interstitial tissue with acute cellular degeneration of epithelial cells.