1Department of Applied Sciences, University of Technology, Baghdad, Iraq
*Corresponding Author: Wissam Lateef Abdullah, Department of Applied Sciences, University of Technology, Baghdad, Iraq, e-mail: wissamla86@gmail.com
Online published on 23 December, 2019.
Analyze sequence of telomerase RNA component (TERC) in Iraqi patient's with breast cancer
17 Iraqi patients with breast cancer included in the current study, their age ranged (17–60 years). The DNA was extracted and TERC gene was amplified by polymerase chain reaction (PCR) using one primer then gel electrophoresis was used to detect the gene, then BLAST software used to analyze the sequence of TERC gene as compared with the reference sequence of this gene in the GenBank.
Eighty mutations found in the sequence of TERC gene. An insertion mutation between the sites 135 and 136 appeared in all patients as compared with the sequence of TERC gene in the GenBank at NCBI database. This mutation was found to be related with breast cancer.
The insertion mutation between the sites 135 and 136 associated with breast cancer
Bioinformatics Method, breast cancer, mutation, telomerase RNA component gene
