Genetic Identification of Aplocheilus Panchax from the Waters of West Papua Using Molecular Approach for Preventing the Spread of Malaria

Aplocheilus panchax domesticated as candidates of broodstock is genetically identified in order to be cultivated and restocked. This research is aimed at identifying the gene of Aplocheilus panchax from the waters of West Papua. Samples were collected from several locations. In Sorong only seven samples were obtained due to the decrease of population of A. panchax, because increase population of predator. The length of the total genomes of A. panchax which were isolated ranged 20 and 30 kb. Their amplicon length was approximately 400 bp. The quality of DNA was excellent with high level of brightness, brighter than the DNA bands in the DNA marker. Cyt b sequences from the samples of KT_MKW_01 with forward and reverse primers were aligned and manually proof read to ensure that DNA sequences corresponded to the peak of electropherogram. The results of BLAST sequence obtained from real sequences from Cyt b gene of A. panchax had 99% similarity with GenBank and alignment indicated that point of polymorphism was not found. All the sequences had exactly similar nucleotide sequences, that the amplified Cyt b gene was a greatly conserved gene and 34 samples were grouped into the same haplotype based on fragment of Cyt b gene.