Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 10

Analysis of Human Platelet Antigen (HPA) in Thrombocytopenia Patients

  • Author:
  • Rachmawati Muhiddin1, Nelly1, Asvin Nurulita1, Tutik Harjianti2, Moch Hatta3, Mansyur Arif1
  • Total Page Count: 6
  • Page Number: 1570 to 1575

1Department of Clinical Pathology, Hasanuddin University, Makassar, Indonesia

2Department of Internal Medicine, Hasanuddin University, Makassar, Indonesia

3Department of Microbiology, Medicine Faculty, Hasanuddin University, Makassar, Indonesia

Online published on 23 December, 2019.

Abstract

Human Platelet Antigen (HPA) is a form of immunogenic polymorphism of platelet membrane glycoproteins. Human Platelet Antigen can cause platelet alloimmunization response will be clinical manifestations of immune thrombocytopenia, such as Fetal Neonatal Allo-immune Thrombocytopenia (FNAIT), Post Transfusion Purpura (PTP), and Platelet Transfusion Refractory (PTR), Idiopathic Thrombocytopenic Purpura (ITP). The aim of this study to research on seroprevalence (genotype and frequency) of Human Platelet Antigen (HPA) in Patients with Non-Immune and immune Thrombocytopenic.

A Cross sectional study was conducted to 66 patients with thrombocytopenia, Samples were collected during the period Mei-July 2019, and HPA genotypes were identified in buffy coat blood samples. Samples were examined by PCR examination to detect HPA genotype at Medical-Research Center Laboratory/Medical Faculty of Hasanuddin University Hospital, Makassar. Data was analysis by the statistical tests used were Independent-t, Mann-Whitney, Chi-Square and Fisher Exact tests. The results of the test were significant statistically if the p-value <0.05.

Result. From 66 patients thrombocytopena, ages 19–82 years,. 41 (62.1%) women and 25 (37.9%) men. 31 (47.0%) patiens were non immune thrombocytopenia and 35 (53.0%) were Immune Thrombocytopenia. The highest HPA frequency (100%) was HPA-1b, 2a, 4a were obtained in both non-immune and immune thrombocytopenia. In this study, there were frequency of HPA 1b, 15a, in both non-immune and immune thrombocytopenia where was not found in the normal population in Makassar (in our research before). Frequency of HPA-4b (2.8%) was obtained in the population of immune thrombocytopenia that was not found in non-immune thrombocytopenia. The Positive distribution HPA-3b was found to be significantly higher in the Immune group (22.9%) than in the Non-Immune group (3.2%) (p <0.05).

The frequency of HPA-3b was found to be significantly higher in immune thrombocytopenia than non-immune thrombocytopenia.

Keywords

Human Platelet Antigen, Non immune, Immune, Thrombocytopenia