Evaluation of Serum Lysyl oxidase and Calreticulin as Biomarkers in patients with Chronic Kidney Disease

This study was carried out to determine the level of lysyl oxidase and calreticulin in CKD patients and correlate the serum level of those markers with routine markers such as urea, creatinine, phosphorus, albumin, duration, stages and causes of disease. A total 65 patient with chronic kidney disease were studied among them (32) female and (33) male patients and (26) healthy as control of them were (13 females, 11 males). Their ages (patients and controls) were ranged from (18–70). Blood samples were collected from patients and control to evaluate serum levels of (serum Urea, Creatinine, calcium, ionized calcium, phosphorus, albumin, lysyl oxidase and calreticulin). The level of lysyl oxidase in patients and control was (19.82 ± 12.96 ng/mL and 27.73 ± 9.48 ng/mL respectively with a significant difference (p=0.013) and the level of calreticulin was patients showed higher serum level of CRT (8.64 ± 7.43 μg/L) than controls (3.46 ± 2.68 μg/L) with highly significant difference (p=0.003). serum urea, Creatinine and phosphorus level was highly significantly increased p value <0.001 in patient CKD as compared to the controls. Where calcium and ionized calcium normal in both groups. The significant decrease of Lysyl oxidase enzyme level in patients with chronic kidney disease and the significant increase of serum calreticulin in patient with chronic kidney disease in advance stages (stage 4 and stage 5). Lysyl oxidase have significant correlation with urea and have no significant correlation with other routine biomarkers used in present study also have no significant relation with duration of dialysis. Calreticulin have significant correlation with phosphorus and have no significant correlation with other routine biomarkers used in present study also have no significant relation with duration of dialysis. Phosphorus have significant negative correlation with albumin and creatinine.