Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 10

Extraction, Purification and Genotyping of β-LactamAse from Local Isolate of Acinetobacter Baumannii

  • Author:
  • Taif Majid Abdul-Hussein1, Hasan Mahmood Abo Almaali2, Essam F. Al-Jumaily1, Shurook M. K. Saadedin1, Wifaq M. Al-Wattar3
  • Total Page Count: 6
  • Page Number: 2013 to 2018

1Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Iraq

2College of Pharmacy, University of Karbala, Iraq

3Department of Microbiology, College of Medicine, University of Baghdad, Iraq

Online published on 23 December, 2019.

Abstract

Beta-lactamase was purified from local isolate of Acinetobacter baumannii by several steps included ion-exchange chromatography by using DEAE-Cellulose and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 11.72, 55.12% respectively. The characterization of the purified Beta-lactamase showed that the molecular weight was about 44, 668 KD as determined by gel filtration. The Acinetobacter baumannii gene (blaTEM) responsible for β-lactamases detection was by PCR. They recorded positive results reaching 39(86.66%), from the isolates.

Keywords

β-Lactamase, Acinetobacter baumannii, Molecular weight, genotyping