1Biomedical Engineering Department, University of Warith Al anbiya'a, Iraq
2Department of Chemistry and Physiology, Veterinary Medicine, Al-Qasim Green University, Iraq
*Corresponding Author: Hamzah H. Kzar Department of Chemistry and Physiology, Veterinary Medicine, Al-Qasim Green University, Iraq, Email: hamza14shukri72@gmail.com
Online published on 23 December, 2019.
Diabetes mellitus (DM) can be describing as a metabolic changes that characterizing by chronic hyperglycaemia and imbalance of carbohydrates, fats and proteins metabolism resulting from disorders in pancreatic secretion of insulin, action of insulin, or both. Development of T2DM can be attributing to the both effects, the genetic an environmental factors. The enzyme encoded by the ALKBH9 gene in human genome that located on chromosome sixteen is Alpha ketoglutarate dependent dioxygenase.
A case-control study of 45 patients with T2DM and 45 healthy controls was conducted at the Al-Qasim hospital in Babylon province. We analyzing the allelic frequency of ALKBH9 genetic polymorphism in study cases. AKDD levels was measuring by ELISA method while SNP of ALKBH9 was investigated by PCR-RFLP method.
The results of this study shown highly significant increase in the levels of serum AKDD in patients group comparing to control group(17.9 ± 1.7 vs. 11.2 ± 1.2). The allelic frequency of A allele was 68% compare to 32% for T allele in patient group. The results of this study showing highly significant differences in levels of AKDD in comparing to risk factors such as ages, genders, smoking, and obesity status(p-value< 0.001). In conclusion, there many risk factors increase the frequency of AA allele comparing to AT, and TT alleles in patients with T2DM in Babylon population, Iraq.
Diabetic mellitus type2, ALKBH9 gene, SNP rs9939609
