1Medico-Legal Directorate, Ministry of Health, Iraq
2Genetic Engineering and Biotechnology Institute, University of Baghdad, Iraq
3Public Central Health Lab, Ministry of Health, Iraq
*Corresponding Author: Abeer Salman Nasseif, Medico-Legal Directorate, Ministry of Health, Iraq, Email: abeeralajely@yahoo.com
Online published on 23 December, 2019.
Mutations that occur in short tandem repeats technique, might lead to alleles mismatched between the child and their parents, which complicate the forensic decisions in Paternity cases. Most of reported microsatellite was confined to single-step mutations. In the present study we reported case of a paternity case processed by 18 loci PowerPlex kit from (Promega) with a mismatch in locus D13S317.
The genotypes of the alleged father, the mother and the child in D13S317 locus were11/12, 8/12 and 8/13, respectively.
These samples were reprocessed with another forensic kit of a different primer which is 24 loci Global Filer from (Applied Bio System) to ensure the mutant alleles. After interpretation of data was made and statistical analysis was calculated in dependable software (Gene Marker HID).
The results stated that the alleged father is the biological father of the child.
Paternity match cases, STR mutation, single-step mutation, multiple-step mutation, forensic decision
