Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 10

Evaluation of Neutrophils Activity and Function in Type II Diabetes Mellitus Patients’ Blood Samples

1Department of Biology, College of Science, Al-Muthanna University, Iraq

2Assistant Prof. Biology Department, College of Science, Muthanna University, Iraq

3Lecturer in the College of Medicine, Al-Muthanna University, Iraq

Online published on 23 December, 2019.

Abstract

Patients with T2DM are considered as immunosuppressed hence they are at risk of infections. Neutrophils are one of the most important phagocytic cells and have important roles in innate immunity. This study was aimed to detect the type of suppression in circulating Neutrophils function in T2DM blood; either resultant from endogenous or exogenous factor.

A total of 60 blood samples were collected from T2DM adult outpatients, and 30 controls or healthy people were involved. Evaluation of neutrophils activity was accomplished for both patients and controls by testing the abilities of circulating neutrophils in blood samples to engulf and reduce NBT dye. Also, IL-8 ELISA test was used to estimate this cytokine levels in patients and controls.

Data from current study showed significant decrease in phagocytic activity and IL-8 levels in T2DM.

T2DM are negatively affected on peripheral Neutrophils via diminishing phagocytic activity and that was due to endogenous and exogenous factors. Hoever, still need more investigations about other related cytokines because it leads to suppression in innate immunity in T2DM patients which predispose them to various infections.

Keywords

Diabetes mellitus, NBT, IL-8, innate immunity, Cytokine, Phagocytosis, neutrophils