1Department of Biology, College of Science for Women, University of Baghdad, Iraq
2Consultant Digestive Surgery, GIT Hospital, FRCS, FACS, Iraq
3M. B. ch. B, Ph D (Path.), College of Dentistry, Private Uruk University, Iraq
Colorectal cancer (CRC) is one of the common malignant tumors of gastrointestinal tract (GIT). The incidence rate of this cancer increased in frequency in the last few years. This study was carried out to investigate the possible association of some risk factors with CRC such as (age, gender, smoking, inflammatory bowel disease, and ulcerative colitis). In this study we analyzed using immunohistochemistry technique of the following markers for 30 selected cases: cytokeratin 20 (CK20), and cytotoxic T-lymphocyte (CD8), the results showed that CD8 and CK20 are positive immunostaining markers.
CD8 expression: Results indicated that CD8 levels were positive in the all cases studied. In term of scores, CRC patients with the score ++ (moderate) represented the highest percentage 14 (46.67%) compared with patients with score+ (weak) 10(33.33%) and score+++ (strong) 6(20.00), the statistical analysis showed increase significant in percentage of patients with score++ as compared with score+ and score+++ at (P<0.05).
CK20 expression: In this study the positive immunohistochemical expression of CK20 was significantly higher in CRC patients with score ++ (moderate) 16 (53.33) in comparison with CRC patients with scores + (weak) 9 (30.00) and score +++ (strong) 5 (16.67) at (P<0.05).
Colorectal cancer, Immunohistochemistry, Cytotoxic T-lymphocyte, Cytokeratin 20
