1Medical City, Baghdad Hospital, Ministry of Health, Iraq
2Ministry of Higher Education and Scientific Research, Middle Technical University, Iraq
3Medical City, Gastroenterology and Hepatology Hospital, Ministry of Health, Iraq
*Corresponding Author: Nawfal Saeed Amen, Medical City, Baghdad Hospital, Ministry of Health, Iraq, Email: riyoma92@gmail.com
Online published on 23 December, 2019.
Analysis of hepatitis delta virus (HDV) isolates from around the world has indicated that there are at least three phylogenetically distinct genotypes with different geographic distributions. The aim of this study was to determine the occurrence of HDV genotypes by direct sequencing in patients with chronic delta hepatitis in Iraq.
A cross-sectional analytical study was done on 364 chronic hepatitis B patients from both Gastroenterology and Hepatology Hospital and Baghdad teaching hospital from August 2017 till August 2018. Serological tests for HDV Ag was measured by ELISA in these patients and confirmed by Nested PCR. All patients with chronic hepatitis B had positive hepatitis B surface antigen for at least 6 months before the study entrance, it confirmed by ELISA for HBs Ag.
Design and methods: Serum samples from 364 chronic hepatitis patients (males to females ratio was 2: 1 with mean age 37 years, range 18—63 years). Samples with positive HDV Ag were undergoing RNA extraction. After reverse transcription, cDNA of partial delta antigen was amplified by nested PCR. The products of the HDV PCR were direction sequenced of 327 bp for HDV gene and it was performed by the National Instrumentation Center for Environmental Management (NICEM), at biotechnology lab. Nucleotide sequences of HDV were compared with previously reported sequences and aligned by using BioEdit
Genotype, Hepatitis delta virus, Sequencing
