1Collage of Health &Medical Technology, Baghdad, Iraq
Endometriosis represents a major medical concern in women of reproductive age. A Reliable single biomarker or panel of biomarkers may help diagnose endometriosis earlier and reduce unnecessary operation. Simple blood tests for prediction and diagnosis of endometriosis have a major impact on women's health. MicroRNAs are small Non-coding RNAs that have been intensively evaluated as biomarkers for several diseases and may hold promise for endometriosis diagnosis. The present study was aimed to explore the possible role of using serum miRNA122 in endometriosis patients to supply a more accurate assessment of the disease and improve the role of other serum biomarkers in endometriosis.
Twenty-five endometriosis patients diagnosed by laparotomy were admitted to the Gynecology Department of Al-Emamain Al-Kademian Medical City, Baghdad, Iraq. A total of twenty-five apparently healthy women were chosen as controls. The miR122 was detected by reverse transcription-quantitative polymerase chain reaction (RT qPCR), while the MCP-1 and TGF-β1 were detected by the ELISA technique.
This study showed that the mean log fold change value of miR122 in plasma of endometriosis patients, which was (19.65), was higher in comparison to controls (3.42) (
Measurements of serum, miRNA122 may be useful in the evaluation of endometriosis patients in combination with other serum markers.
Endometriosis, MicroRNA122, MCP-1, TGF-β1, PCR
