Indian Journal of Public Health Research & Development

One of the most consumed crops globally is bread wheat (Triticum aestivum). This crop possesses a hexaploid genome that makes it possible to manipulate its genome and produce new mixtures of desirable traits. However, the fertility of bread wheat is highly affected by heat stress occasioned by global warming. In this study, wheat spikes RNA was extracted and used for cDNA synthesis. The conventional PCR approach was used to amplify some gene prior to gene cloning and determination of genes expression via quantitative PCR. The differences in genes expression were determined over a range of temperatures. Heat stress effect on the activity of meiotic genes, transcription factors, and chromatin organization was showcased in the varying levels of genes expression at the studied temperatures.