1Middle Technical University, College of Health & Medical Technology, Baghdad, Iraq
2Al-Nahrain University, College of Biotechnology, Department of Molecular and Medical Biotechnology, Baghdad, Iraq
Online published on 27 March, 2020.
Klebicins (bacteriocins) are natural antimicrobial peptides that produced by ribosomes of Klebsiella species. The fatal action of klebicin is directed to closely related bacteria to the producing strains. The present study aims todetect theklebicin genecluster in Klebsiella pneumoniae isolates.Thirty one K. pneumoniaisolates were specified by biochemical and microbiological methods, eventually confirmed by API 20E system and VITEK-2 system. Antibiotic susceptibility test was done five antibiotics (Amikacin, Ampicillin, Augmentin, Azetreonam and Tetracycline). DNAextraction was carried out for 31 K. pneumonia isolates andklebicingene cluster was amplified from extracted DNA by PCR technique. Results of current study were shown that the resistance rate of studied antibiotics was registered as Amikacin 32.26%, Ampicillin 64.52%, Augmentin 51.61%, Azetreonam48.39% and Tetracycline 41.94%.Amplification results revealed that Klebicin gene cluster was isolated from15(48.39%) of K. pneumoniae isolates.
Bacteriocin, Klebicin, Antibacterial agents, Klebsiellapneumoniae
