1Assistant Lecturer, Faculty of Dentistry, Misr University for Science and Technology
2Professor of Endodontics, Faculty of Dentistry, Beni Suef University
3Associate Professor of Endodontics, Faculty of Dentistry, Minia University
Online published on 27 March, 2020.
This study was performed to evaluate the effect of three intracanal edicament Conventional calcium hydroxide (Ca(OH)2), Ca(OH)2 nanoparticle and Combined Ca(OH)2 with silver nanoparticle on E.faecalis bacterial biofilm within infected root canals. Forty five extracted human single rooted teeth were sterilized followed by mechanical preparation Microbiological culturing of E. faecalis was performed and incubated for 7 days. After incubation period, proper irrigation and premedication bacteriological sample was taken teeth were randomly divided according to the intracanal medicament used, samples were incubated in 37°C for 7 days. Second microbiological sample was taken after 7 days and culturing was done to allow bacterial count reduction. The non-parametric Kruskal-Wallis test was performed to evaluate the bacterial count reduction and percent of reduction at significance level was set to p ≤ 0.05.
The antibacterial effect of combination of calcium hydroxide with silver nanoparticle was better than calcium hydroxide nanoparticle and followed by Conventional calcium hydroxide against E. faecalis bacteria.
Combination Ca(OH)2 with silver nanoparticle can be used instead of Conventional Ca(OH)2 against E. faecalis bacteria in necrotic root canals.
Calcium hydroxide, E. faecalis, Silver nanoparticle, calcium hydroxide nanoparticle
