Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 11

High Level Aminoglycoside Resistance in Clinical Isolates of Enterococci

  • Author:
  • Alexander Kiruthiga1,2, Kesavaram Padmavathy3,, Praveen Shabana4, Sumathi Gnanadesikan5, Jeevan Malaiyan6
  • Total Page Count: 4
  • Page Number: 2938 to 2941

1Research Scholar, Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, BIHER, Chennai

2Senior Lecturer, Department of Microbiology, Priyadarshini Dental College and Hospital, Pandur, Thiruvallur

3Associate Professor, Department of Microbiology, Research Laboratory for Oral and Systemic Health, Sree Balaji Dental College and Hospital, BIHER, Chennai

4Head, Department of Laboratory Medicine, Meitra Hospital, Calicut

5Professor & Head, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute, Chennai

6Associate Professor, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute, Chennai, India

*Corresponding Author: Kesavaram Padmavathy, Ph. D., Department of Microbiology, Sree Balaji Dental College and Hospital, BIHER, Velachery Main Road, Chennai. India-600100, Phone No.: 91-9884164212, e-mail: padmabakianath@gmail.com

Online published on 27 March, 2020.

Abstract

Enterococci though being intestinal commensal flora have gained significance as a serious nosocomial pathogen owing to their exceptional ability to survive in the harsh environments and increasing high level resistance to antibiotics. The emergence of high level aminoglycoside resistant (HLAR) clinical enterococcal isolates is of serious concern worldwide and thwarts the available therapeutic options.

A total of 25 non-repetitive isolates of Enterococci (E. faecalis (n= 15), E. faecium (n= 10)) recovered various clinical samples were screened for HLAR among the isolates was performed by disk diffusion method using High Level Gentamicin and High Level Streptomycin disks. The isolates were further confirmed as HLGR and HLSR by agar dilution method. Genes encoding Aminoglycoside Modifying Enzymes (AGMEs) were detected by multiplex PCR. Susceptibility to linezolid was determined by Kirby Bauer disk diffusion method.

All the 25 isolates of enterococci exhibited HLAR phenotype (resistant to HLG and/or HLS). Majority (96%) of the isolates were resistant to HLG and 64% were resistant to HLS. MIC of gentamicin was >500 μg/mL for HLGR isolates and MIC of streptomycin was MIC > 2000 μg/mL for HLSR isolates. In our study, 92% of the enterococci harbored aac(6’)-Ie-aph(2’’)-Ia and/or aph(3’)-IIIa. Of note, 15(60%) of the enterococci exhibited dual resistance to gentamicin and streptomycin (HLGRHLSR). Nevertheless, 92% of the isolates were found to be susceptible to linezolid.

Prompt detection and characterization of HLAR among clinical strains of Enterococci within our setting is very essential as few of them exhibit co-resistance to glycopeptides and have lost synergism with the cell wall active agents.

Keywords

AGMEs, E. faecalis, E. faecium, HLAR, HLG, HLS