1Post Graduate Student, Department of Oral Pathology and Microbiology, SreeBalaji Dental College and Hospital, Bharath Institute of Higher Education and Research
2Professor, Department of Oral Pathology and Microbiology, SreeBalaji Dental College and Hospital, Bharath Institute of Higher Education and Research
3Reader, Department of Oral Pathology and Microbiology, SreeBalaji Dental College and Hospital, Bharath Institute of Higher Education and Research
4Professor and Head of the Department Department of Oral Pathology and Microbiology, SreeBalaji Dental College and Hospital, Bharath Institute of Higher Education and Research
Conventional tissue fixation and processing is as old as 100 years and still remains the gold standard in which all new technologies and methods are need to be assessed. Tissue processing is one of the important steps for obtaining good thin sections without artifacts. Though conventional tissue-processing methods are most commonly followed, they are well-known as very laborious and tedious procedures. Microwaves is a form of electromagnetic wave-induced heat, when applied in histotechnology, reproducibly yields histolologic material of similar or superior quality than conventional processing methods, making it popular in the recent years. Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.
Tissue processing, Microwave, Staining