1Post Graduate, Department of Microbiology, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai
2Professor, Department of Microbiology, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai
*Corresponding Author: Dr. Kiran Madhusudhan, Professor, Department of Microbiology, Sree Balaji Medical College and Hospital, Bharath Institute of Higher Education and Research, Chennai-73., M No- 9841434966, E Mail- brsmadhu@yahoo.co.in
Online published on 27 March, 2020.
Thyroid cancer is the most common endocrine neoplasm. Different proliferation markers such as Ki67, proliferating cell nuclear antigen (PCNA) and argyrophilicnucleolar organizer regions (AgNORs) have been examined and observed their importance in thyroid tumours. It has also been reported that like other markers, AgNORs are also related to the rate of cell proliferative activity. As it is also found useful in distinction, it can also be used in distinguishing different lesions.
60 gross specimens were taken for study. These biopsy specimens were routinely fixed in 10% formalin and processed in paraffin wax. 5 microns thick Sections were cut and taken for the study and hematoxylin and eosin staining of sections was done. Histopathologicalexamination of these sections were done. Special stain (AgNOR Stain) procedure was also done in 60 cases.
The present study showed that mean mAgNOR in case of nodular goitre was 1.59 (SD ± 0.13) with a range of 1.4–1.75, in benign lesions i.e.; Follicular Adenoma/Hurthle cell Adenoma was 3.63(SD ±0.41) with a range of 2.9–4.05, in malignant lesions i.e.; in papillary carcinoma was 2.77 (SD ± 0.56) with a range of 1.5–4.05 and in follicular carcinoma was 4.92 (SD± 0.27) with a range of 4.68–5.21.
Our study concluded that mAgNOR count and pAgNOR values can differentiate follicular adenoma and papillary carcinoma and also can differentiate non-neoplastic, benign and malignant lesions of thyroid.
mAgNOR, pAgNOR, PTC, FTC, FA, NG, H&E
