Antimicrobial Activity and Surface Characterization of a Denture Relining Material Coated with Reynoutria Elliptica Extract

The purpose of this study was to evaluate the antimicrobial effect and surface characterization of a denture relining material coated with Reynoutria elliptica extract.

A denture relining disk was coated with coating resin containing Reynoutria elliptica extract. Extracts of coated specimens were analyzed to determine polyphenol content, and also incubated with S. mutans and C. albicans for 48 h. The coated surfaces were evaluated for microhardness, contact angle, and color characteristics. Cell viability was confirmed by performing MTT assay according to ISO 10993-5. All data were checked with one-way ANOVA followed by the Tukey's multiple comparison test (p=0.05).

The antimicrobial activity of experimental groups was significantly higher than control group on both S. mutans and C. albicans. Extracts analysis revealed that the level of polyphenol released from the experimental groups increased with increasing proportion of added Reynoutria elliptica extract. The surface microhardness, △E* values, and cell viability were not significantly different between experimental groups and control group. The contact angle test revealed that the experimental groups exhibited more hydrophilic surface than the control group.

Coating materials containing Reynoutria elliptica extract can be used as antimicrobial dental materials, with no deterioration of surface characteristics and biocompatibility.