RAPD Identification of Bacteria Isolated from Arm Third Degree Burn Wound

This study aims to isolate different types of bacteria from the wounds of the third degree burns in the forearm area and to define those types at the morphological level and using the RAPD-PCR technique. And study the sensitivity of isolated species towards certain types of antibiotics traded. 35 swabs of third-degree burns were collected in the forearm area of ​​35 patients. 12 species of bacteria were isolated and purified from those swabs. The necessary tariff tests were carried out.

The isolated bacteria were identified as morphology and the result was the following: Bacillus sp, Enterobacter sp, E. Coli sp, Klebseilla sp, Pseudomonas aeruginosa and MRStaphylococcus aureus. Seven antibiotics; amoxicillin, Cefaclor, ampicillin, amoxicillin/clavulonic acid, Ciprofloxacin, Imepenim and Vancomycin were tested to identify the isolates of the isolated bacteria. DNA was extracted from isolates. The RAPD-PCR method was applied to DNA extracts according to a standard method using 3 primers. This technique resulted in repeated trip patterns used for genetic differentiation. The phylogeny tree in Figure 2 obtained from statistical analysis of the RAPD-PCR band pattern of 12 bacterial isolates using three RAPD primer shows that there are only two main groups branched from one ancestor at linkage distance 16%. One of these groups contains isolate number 10, while the other group contains isolate no.12 and branched at linkage distance 15% to other two sub groups one of them contains isolates 1, 11 while the other group contains the other remaining 8 isolates. The study showed different types of infectious bacteria according to the age of the injured and the causes of injury, which is worth studying on a larger scale.