1Professor, Biochemistry, College of Dentistry, University of Baghdad, Iraq
2Assistant Lecture, Biochemistry, College of Dentistry, University of Baghdad, Iraq
3Biological, Basic Science Dep., College of Dentistry, University of Baghdad, Iraq
Online published on 8 March, 2019.
Enzyme activities can be utilized as good indicators for diagnostic purposes. Thus, determination of kinetic parameters of the target enzymes facilitates the interpretation of the effect that may be occurred on activities of the studied enzymes. The aim of this study was to determine of Vmax and Km of salivary lactate dehydrogenase (LDH) enzyme in presence and absence of the titanium dioxide nanoparticles (TiO2 NPs).
The structural properties of (TiO2 NPs) have been determined using UV-Vis spectrophotometer and SEM. TiO2 NPs and sharp round peaks around 220nm with a spherical shape and average particle size <50 nm. Results: Km and Vmax values of LDH in absence and presence of TiO2 NPs were found to be 0.02mmol/L, 15.6U/L and 0.01mmol/L, 17.2U/L, respectively. Conclusion: our results confirmed the activating effects of TiO2 NPs on salivary LDH activity. The effects of TiO2 NPs on salivary LDH activity may be due to the activity of this NPs type and/or the conformational changes that can be occurred on the protein structure of the enzyme after interaction with these NPs.
TiO2 nanoparticles, LDH activity, saliva, Km, Vmax, kinetic study
