1Department of Pathology and Forensic Medicine, Mustansiriyah Universitty, College of Medicine, Iraq
2Department of Pathology and Forensic Medicine, Mustansiriyah University, College of Medicine, Iraq
3Ibn Sena University of Medical and Pharmaceutical Sciences, Baghdad, Iraq
Online published on 8 March, 2019.
Background: Differentiation of endometrial hyperplasia with atypia from well-differentiated endometrial endometrioid adenocarcinoma in endometrial biopsies is a difficult task. The final diagnosis may be achieved after hysterectomy. Non-invasive methods are limited, but the new advances of molecular and tumor genetics, especially after discovery of microRNAs, have opened a new era in the diagnosis and management of tumors. MicroRNAs were found to target genes that control cell cycle, DNA repair, apoptosis, angiogenesis and so on. Therefore, aim of current study was to study expressions of microRNA genes, miR-93and miR-301a, by qRT-PCR in samples taken from patients with endometrial endometrioid adenocarcinoma, endometrial hyperplasia with atypia and normal endometrial tissues. Methods: Participants were dividedn into three groups of 38 subjects each. Histopathological diagnosis of hysterectomy samples was performed as gold standard method. Small RNAs extracts were obtained by miRNeasy FFPE Kit-QIAGEN. Quantity and purity of small RNAs were determined by nanodrop spectrophotometer. Then, obtained amplicons were reverse transcribed into corresponding cDNAs and amplified in triplicates by qRT-PCR using TagMan® MicroRNAs amplification kit and primers from Applied Biosystems. The mean Ct values of each specimen were taken for statistical analysis and gene expression studies. Results: Data from current study showed significant overexpression of microRNAs-93 and miR-301a in samples taken from patient with endometrial endometrioid adenocarcinoma compared to atypical endometrial hyperplasia with sensitivity of 92.11% and 94.74%, for miR-301a and miR-93, and 97.36% for both genes, respectively. The specificity for both genes was 100. Accuracy, for miR-301a and miR-93, was 94.74%, and 97.36%, respectively, and for both genes was 92.11. Conclusion: Cases of endometrial biopsies with qRT-PCR expression of 1.5 or more of miR-301a and/or miR-93 genes are considered as well differentiated endometrioid endometrial adenocarcinoma. Cases with expression of <1.5 are considered as endometrial hyperplasia with atypia.
Endometrioid adenocarcinoma, endometrial hyperplasia with atypia, microRNA, microRNAs-93, miR-301a
