1Department of Applied Sciences, University of Technology, Baghdad, Iraq
2Department of Business Economics, Al-Nahrain University, Baghdad, Iraq
*Corresponding Author: Ammar Adil Jasim, Department of Applied Sciences, University of Technology, Baghdad, Iraq, Email: ammar.adel8585@gmail.com
Online published on 20 March, 2019.
The present study aimed to use bioinformatics methods for analysis function of Beta 2 microglobulin (B2M) protein in patients with prostate cancer.
This present study included 10 patients with prostate cancer. The human DNA was extracted and the three exons of B2M gene were amplified using three primers.
The results revealed 24 mutations in the sequence of exon 1 and exon 2 while in exon 3 for B2M gene of patients did not determined a mutations. In exon 1, appeared a point mutation at the position 44, 711, 557 in more samples of patients. Also in exon 2, appeared a point mutation at the position 44, 715, 448 in more samples of patients, these mutations recorded in ENA, DDBJ and GenBank at NCBI databases with the number LC424499 and LC424500, respectively. The results showed using STRING program that the mutations had effect on the function of B2M protein for patients compared to B2M retrieved from NCBI.
A point mutations were determined in exon 1 or exon 2 of B2M gene at the site 44711557 or site 44715448 respectively, may be considered as the cause of prostate cancer. The mutations in B2M gene sequence affected the function of B2M protein by lost its linked with HLA-1.
B2M gene, Bioinformatics Methods, Prostate Cancer, STRING program, point mutation
