Effectiveness of Disinfectant A and B on the Growth of Bacteria in the Area of Central Surgical Installation of Hospital X in Kudus City

There is a limit of using centralized AC in surgical rooms that requires hospital to control the growth of microorganism by using disinfectant A. Disinfectant A has been used for so long and has tested for its effectiveness, so hospital X held effectiveness test by comparing disinfectant A with other disinfectant. The aim was to analyze the effectiveness of disinfectant A and B to the growth of bacteria in Central Surgery Unit Hospital X Kudus City.

The research was a quasi-experimental research with one group pre-test post-test. The number of samples was 36 samples. Data collection was conducted with laboratory observation. The data were then analyzed by Wilcoxon Test.

Statistical result shows that there is no significant difference on the effectiveness of disinfectant A and B in surgical room (p > 0, 05) for both centralized and split AC. There are three identified bacteria in surgery room namely Staph. Aureus, Staph. Epidermidis dan Baccilus Sp. From the observation, the number of bacteria before using disinfectant A and B in rooms with split AC was Staph. Epidermidis 0, 66 CFU/cm², Staph. Aureus 0, 67 CFU/cm² and Baccilus Sp 1, 67 CFU/cm², While in rooms with centralized AC, the observed number of bacteria can be seen as follows: Staph. Epidermidis 0, 66 CFU/cm², Staph. Aureus 0, 33 CFU/cm² and Baccilus Sp 1, 67 CFU/cm². After 20^th and 240^th minute using disinfectant A and B, in rooms with centralized and split AC, there was no growth of bacteria (−).

The observation on the effectiveness of disinfectant A and B shows no growth of bacteria on the 20^th and 240^th minute which means that disinfectant B can be the alternative of disinfectant in surgery room.