Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 4

Involvement of KRAS rs61764370 T>G Gene Variation in Leukemia Patients of Saudi Arabia

  • Author:
  • Osama Al-Amer1,, KF Alsharif2, Rashid Mir1, FM Abu-Duhier1, Abdulraheem Almalki2, Wayil Yassen3
  • Total Page Count: 6
  • Page Number: 357 to 362

1Department of Medical Laboratory Technology, Prince Fahd Bin Sultan Research Chair, Faculty of Applied Medical Sciences, University of Tabuk, Kingdom of Saudi Arabia

2Departments of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Taif University, Kingdom of Saudi Arabia

3Departments of Oncology, King Salman Armed Forces Hospital, Tabuk, Kingdom of Saudi Arabia

*Corresponding author: Dr Osama Al-Amer, Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Kingdom of Saudi Arabia.

Online published on 6 April, 2019.

Abstract

Recently, a variant allele in the 3′UTR of the KRAS gene (rs61764370 T>G) was shown to be associated with an increased risk for developing multiple cancer types like lung, ovarian and breast cancer. Therefore, we investigated the association of KRAS (rs61764370 T>G) gene variation in leukemia susceptibility in our population.

This population-based case-control study was done on 72 clinically confirmed Leukemia patients and 87 matched healthy controls with no history of any type of cancer. The KRAS rs61764370T>G genotyping was detected by using Allele specific PCR.

We observed a statistically significant difference in the frequencies of KRAS TT, GT and TT genotypes among patients and healthy controls (p=0.0271). This study reported significantly higher percentage of TT (11%), GT (75%) and GG (14%) genotypes in patients compared to controls TT (25%), GT (69%) and GG (06%) genotypes while higher percentage of GG (14%) genotype were reported in patients compared to control GG (6%) genotypes. Our findings showed that the KRAS rs61764370 T>G variant was associated with an increased risk of Leukemia in codominant inheritance model (OR=5.5, 95%CI= (1.43–21.0); RR=2.20, (1.04–4.64), p=0.038 TT vs GG. Dominant inheritance model (OR=2.70, 95% CI= (1.21–6.52): RR=1.45(1.10–1.91), P=0.007 (TG+ GG) VS TT and Recessive inheritance model (OR=2.64, 95%CI= (0.86–8.13), RR (1.70(0.82–3.54) P=0.025 (GG vs TT+GT) tested. While, the G allele significantly increased the risk of Leukemia (OR= 1.57; 95% CI= (1.03–2.45); RR 1.22(0.99–1.51) P=0.047 compared to T allele.

Our findings indicated that KRAS rs61764370 GG genotype and G allele are associated with an increased susceptibility to leukemia in Saudi Arabian population. It can be used as a predisposing genetic marker for Leukemia. Furthers studies with larger sample sizes are necessary to confirm our findings.

Keywords

KRAS rs61764370, SNP-Single-nucleotide polymorphism, Leukemia, OR-Odds ratio, CI-Confidence interval