Estimation of TLR-4 and Cytokines Levels (Interleukin-1 Beta, Interleukin-8, and Tumor Necrosis Factor) in Serum and Peritoneal Fluid of Endometriosis Women

Many women have a delay in diagnosis of endometriosis; an important factor that contributes to the diagnostic delay is the lack of noninvasive methods for detecting the disease. The aim of this study was to evaluate the levels of IL-1β, IL-8, TNF-α, and TLR-4 in serum and peritoneal fluid (PF) of women with endometriosis. The case group comprised of 30 women with endometriosis who newly diagnosed (admitted to the Gynecology Department of Al-Emamain Al-Kademian Medical City, Al-Zahraa teaching hospital and Al-kut hospital of pediatric and obstetric) and 30 control group during the period from November 2016 to November 2017. The four biomarkers were measured by using ELISA. Endometriosis patients had significantly higher serum and PF concentrations of the four biomarkers compared to controls. These results may indicate the potential role of these biomarkers in non-invasive diagnosis of endometriosis.