1Assistant Professor, Department of Pediatric Dentistry, Dankook University, Korea
2Fellow, Department of Pediatric Dentistry, Dankook University, Korea
3Associate Professor, Department of Dental Hygiene, Sunmoon University, Korea
4Assistant Professor, Department of Pediatric Dentistry, Wonkwang University, Korea
5Assistant Professor, Dept. of Dental Hygiene, Kyungbok University, Korea
6Professor, Department of Pediatric Dentistry, Dankook University, Korea
The aim of this study was to evaluate the potential of stemness of the human dental pulp cells from supplemental tooth during the extended culture.
Supplemental tooth was collected from a patient who was 5-year-old and soon after extraction of the tooth, the primary cells were cultured from the pulp tissues. Those were subcultured until 16th passage and each passage cells were evaluated their stemness with expression of cell surface markers by using flow cytometric analysis.
The cell surface markers which are representative mesenchymal stem cell markers, such as CD44, CD73, CD90 and CD146 were expressed during the whole passages. The expression of these markers showed second peak at 6–7 passages and slightly decreased after 8th passage during the extended culture of dental pulp cells from this supplemental tooth. In addition, those cells were confirmed with negative expressions of CD24, CD146 markers. The proliferation rate of stem cells from the pulp of supplemental tooth was two to three times fast comparing to the stem cells from other dental pulp tissue.
Pulp tissue in supplemental tooth could be a choice for donor site of stem cells since it was confirmed that they possessed stemness, especially strong in early passages.
Stem cells, Supernumerary tooth, Supplemental tooth, Flow cytometry, Extended culture
