Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 5

Evaluation the Antimicrobial Efficacy of NaOCL-EDTA, MTAD and NaOCL-citric Acid Against Enterococcus faecalis in Root Canals of Primary Teeth (an in vitro study)

1Master Student, Department of Pedodontics and Preventive Dentistry, College of Dentistry, University of Baghdad, Iraq

2Assistant Professor, Department of Pedodontics and Preventive Dentistry, College of Dentistry, University of Baghdad, Iraq

Abstract

The main etiology of pulp diseases and per-radicular lesion is presence of microorganisms. The disinfection phase of root canal therapy is fundamental determinant in complete healing of periapical tissue. The purpose of this study was to compare the antimicrobial efficacy of 3% NaOCL-17%EDTA, MTAD and 3% NaOCL-10% citric acid against Enterococcus faecalis.

This study utilized 45 human primary teeth with single root. The samples infected with E. faecalis for 2 days. Incubation all the infected samples in brain heart infusion broth (for 2 weeks). before irrigation procedure swaps from all the canals were taken and considered as controls. Post irrigation samples were taken after complete treatment. Evaluation the disinfection efficacy of three methods was done by cfu/ml to show the number of the remaining bacteria, statistical analysis was done using one way anova and post-hoc Dennett t3 test.

All samples treated with 3% NaOCL-17% treated with 3% NaOCL and 17% EDTA showed nearly complete absence of bacteria. The 15 samples treated with MTAD showed similar results to the first group, while the least antibacterial effect was seen in the 3% NaOCL-10% citric acid group. Statistical analysis of data showed highly significant difference between groups (P< 0.01).

This study showed that both 3% NaOCL-17% EDTA and MTAD are effective against E. faecalis, while 3% NaOCL and 10% citric acid showed the least effectiveness.

Keywords

NaOCL, EDTA, MTAD, citric acid, Enterococcus faecalis. BHI