Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 6

MicroRNA-146a Gene Polymorphism is Associated with an Increased Susceptibility to Lung Cancer Disease: A Case-control Study

1Department of Medical Lab Technology, Faculty of Applied Medical Sciences, Prince Fahd Bin Sultan Research Chair, University of Tabuk, Kingdom of Saudi Arabia

*Corresponding Author: Dr. Jamsheed Javid, Department of Medical Lab Technology, Faculty of Applied Medical Sciences, University of Tabuk, Kingdom of Saudi Arabia

Online published on 11 July, 2019.

Abstract

Genetic variability in miRNA-146a (rs2910164C>G) has a significant influence on the expression and secondary structures of the miRNA-146a. Presently, very limited evidences are available to associate the miRNA-146a (rs2910164C>G) polymorphism and susceptibility to lung cancer. In the present study, we aimed to find out the association of miRNA-146a (rs2910164C>G) polymorphism with the susceptibility to develop lung cancer.

Present case-control study included histopathologically confirmed lung cancer patients (n=80) and healthy controls (n=80). Genotyping of miRNA-146a (rs2910164C>G) polymorphism was done by allele specific PCR technique.

It was observed that there was a statistically significant difference in the frequency of miRNA-146a (rs2910164 GG, CG and CC) genotypes between lung cancer patients and healthy controls (p=0.0097). Among the two groups, homozygous miRNA-146a CC genotype was overrepresented by lung cancer patients than healthy controls (40% vs. 18.75% respectively). Compared to miRNA-146a GG genotype, odds ratios of 4.3 (95%CI=1.24–14.69, p=0.036) was observed for the homozygous miRNA-146a CC genotypes. In addition, it was observed that all the lung cancer stages (I to IV) and histological grades (Grades 1 to 3) represented higher miRNA-146a C allele frequency. Squamous cell carcinoma patients represented much higher frequency of miRNA-146a C allele than adenocarcinoma patients (SCC=0.78 & ADC= 0.63).

In conclusion, the genetic variability in miRNA-146a gene may be associated with the susceptibility to lung cancer disease. The risk of developing lung cancer disease was 4.3 times more in association with the CC genotype of the miR-146a (rs2910164C>G) polymorphism.

Keywords

Lung Cancer, susceptibility and miRNA-146a gene polymorphism