1Department of Horticulture and Land Escaping, College of Agriculture and Marshes, University of Thi-Qar, Iraq
*Corresponding Author: Ahmed D. Khaleaf Al-Asadi, Department of Horticulture and Land Escaping, College of Agriculture and Marshes, University of Thi-Qar, Iraq, Email: dr.ahmedalasdi8@gmail.com
Online published on 11 July, 2019.
The salinity is one of the most important problems facing agriculture in the world, especially Iraq. This study is concerned with examining the effect of four sodium chloride concentrations (0, 25, 50 and 75 mM) and four trehalose sugar concentrations (0, 5, 10 and 15 gm. L−1) in some chemical characteristics of somatic embryos of date palm c.v Um-Aldehine in vitro. The results showed: That the increasing in sodium chloride concentration in culture media had a significant increase in the concentrations of carbohydrates and proline. In addition, the treatment (75 mM) of sodium chloride achieved the highest averages, also it has led to a significant decrease in proteins percentage. The effects of increasing trehalose sugar concentration in culture media has significantly increased the carbohydrates, proline and proteins. However, the treatment of (15 g. L-1) resulted the highest averages. The interaction treatment between sodium chloride (75 mM) and trehalose sugar (15 g. L-1) has significantly increased the carbohydrates and proline. The added concentrations of trehalose sugar to the culture media contributed to decreased the effects of salt stress, therefore, increased the tolerance of somatic embryos of date palm to the salt stress.
Sodium chloride, Trehalose sugar, Somatic embryos, Date palm, In vitro
