1Dept. of Microbiology, ENT, College of Medicine, University of Babylon, Iraq
2Dept. of Surgery, ENT, College of Medicine, University of Babylon, Iraq
*Corresponding Author: Ilham A. Bunyan Dept. of Microbiology, College of Medicine, University of Babylon, Iraq, Email: Ilhamalsaedi2008@gmail.com
Online published on 11 July, 2019.
Sore throat, also known as throat pain, is pain or irritation of the throat. It is usually caused by pharyngitis or tonsillitis. These infections are usually self-limiting with symptoms typically resolving within 1–2 weeks and complications are rare for the vast majority of throat infections. The present study was aimed to isolation of bacteria from throat infections, identification by routine bacteriological, biochemical tests, vitek, API20, and molecular detection of bacteria by using PCR technique with use of specific primers.
The present study included, (204) samples which were collected from patients with throat infections, who were visiting AL-Hilla Teaching Hospital and private clinic of superadviser for the period from February to December (2017). Two swabs were collected one for culturing and the other for direct DNA extraction for swabs of Moraxiella catarrhlis and Haemophilus influenza.
Only 190(93.1%) were positive cultures; whereas 14(6.9%) samples showed no bacterial growth. Gram-positive bacteria constituted 116/190(61.1%) from the total isolates and Gram-negative bacteria constituted 74/190(38.9%). Streptococcus pyogenes was appeared with percentage 78(67.2%), and Staphylococcus aurous constituted 38(32.8%), and out of the 74(100%) the Moraxiella catarrahllis was the most predominant as gram negative bacteria with percentage 44(60%), Klebsiella pneumonia was 12(16%), E. coli appeared with 10(13%) and Haemophilus influenzae had 8(11%) positive isolates.
Throat infections, DNA extraction, Epi20E, Haemophilus influenza, PCR
